Abstarct: In This Research, we analysis the vibration of hyperelastic of microtube which contains flow conduct in the modeling of microtube to consider a model. Near to the real model, we studied the modern microarrays of hyperelastic in which the relation of strain stress is nonlinear we added the waterspland used the model Yeo. These modeles forcast the lastic action and nonlinear relationship of strain stress in materials. And we can involve them to study the nonlinear factor and other factors. So, According to thory thyravilber to we studied the vibration of microtube and hyperelastic model in condition with border support of two joints. To extract the equation of system we used theory of thyravilber to and principle of Hamilton. First, we should compute the Kinetic energy and potential energy, then we should dimension the equation of system and gain the border conditions of dimension we change the vibration equations of system to differential and normal equation by using galerkin method. First we study the sustain ability of system and normal frequency and the speed, length and thickness of flow. To solve nonlinear equations we use analytical disturbances and multiple scale. We draw the chart the effect of speed on nonlinear frequencies.