Abstarct: Antibiotics are one of the most important environmental micro pollutants. The aim of the present study was to investigate the adsorption process of levofloxacin antibiotic from aqueous solution using raw sepiolite, sepiolite modified with manganese nano oxide and manganese oxide nanoparticles in batch system and optimization of the adsorption by Taguchi design method. The properties of the adsorbents were determined by SEM, FTIR, XRD and EDX analyzes. Five steps of the experiments including optimization of solution pH, contact time, adsorbent dosage, initial concentration of levofloxacin and ambient temperature were performed to obtain effective factors in removing levofloxacin. The results showed that adsorption of raw sepiolite strongly depends on the pH of the medium and modification of this adsorbent with MnO2 decreased the dependence of the adsorbent on pH. The results of the Taguchi design method confirmed the results of these tests. The adsorption of all three adsorbents followed the Freundlich isotherm model and the pseudo-second-order kinetic model. Also, considering the thermodynamic properties of the adsorption, it can be concluded that the adsorption process of levofloxacin by the sepiolite adsorbent was Exothermic and by two modified sepiolite adsorbents and manganese oxide nanoparticles were endothermic. The amount of adsorption energy showed physical adsorption by all three adsorbents. According to SEM images, the adsorbents showed lower porosity morphology after levofloxacin adsorption. Also, the particle size of all three adsorbents remained in the range of nanometer after adsorption. The results of EDX and FTIR analysis of the adsorbents after the adsorption process showed the presence of carbon element in all three adsorbents due to the adsorption of levofloxacin.