Abstarct: Sustainable agriculture with increasing agricultural yields and community health care is one of the most agricultural researcher's goals. In recent decades; application of chemical fertilizers as well as other chemical inputs caused numerous environmental problems such as soil and water contamination; fertility reduce and loss of chemical balance in soil. In these cases, biological and chemical soil modifiers are highly recommended. Accordingly, an experiment was conducted to evaluate the effects of zeolite and micohrriza on plant uptake and leaching of nitrogen and phosphorus in the soil under Trifolium alexandrium cultivation. A factorial randomized complete block design with four replications was performed under greenhouse conditions including two sterile and non-sterile soil treatments. Factors were tested including two micohrriza levels (with and without Glomus intraradices inoculation) and two zeolite levels (zero and 50 g kg-1 soil) and three nutrients levels including nitrogen (143 mg ammonia nitrate in each column equal to 280 kg ha-1; as well as phosphorus levels (1100 mg potassium phosphate in each column equal to 485 kg ha-1) and also control treatment. The results showed that the application of micohrriza had significant effect on plant height, root dry weight and shoot dry weight but zeolite alone had no significant effect on these characteristics. However, its co-application with mycorrhiza was a positive effect on some of these characteristics. Application of zeolites decreased percent root colonization. But the percentage of root colonization increased with increasing nitrogen and phosphorus fertilizer. Mycorrhiza inoculation and application of zeolite, had significant effect on reducing the leaching of nitrogen and phosphorus and their storage in soil. The other results also showed that the effects of double and triple-tested factors were significant on growth characteristics, nitrogen and phosphorus in soil and leaching of soluble. Regarding to the proper function of mycorrhiza and zeolite to reduce nitrogen and phosphorus leaching, it can be concluded that mycorrhiza and zeolites, especially with phosphorus and nitrogen in chemical fertilizers, can cause positive effects on traits. In fact, according to these results, the use of mycorrhiza as a bio-fertilizer instead of chemical fertilizers, can reduce the leaching of these elements. In general, mycorrhiza can be used as a replacement for chemical fertilizers (especially in symbiotic plants). Appropriate amounts of nitrogen and phosphorus fertilizers along with biological fertilizer and zeolite, could increase yield compared to the chemical fertilizers alone. The results could be useful to reduce fertilizer applications, decrease production cost and to prevent contamination of soil and groundwater.