Abstarct: In most soils of Iran, zinc deficiency is one of the factors that reduce the growth and yield of crops. Mycorrhizal inoculation plays an important role in disrupting the antagonistic effect of phosphorus and zinc in the rhizosphere and promptly zinc foliar application can contribute to the availability of zinc in the plant. Therefore, this study aimed to investigate the effect of mycorrhiza and zinc foliar application on morphological, physiological and yield traits of two barley cultivars in two pot and field experiments. Both pot and field experiments were conducted in a randomized complete block design with three replications at Chanaran Gardening and Agriculture Research in three replications and the pots were placed in the same farm conditions at the same farm location. Experimental factors included Yusuf and Julgeh as first factor, mycorrhizal fungi as second factor in three control, Glomus mosseae and Glomus intraradices levels and zinc foliar application as third factor in four levels including control, zinc oxide nanoparticles, ordinary zinc oxide and nano mixtures with ordinary zinc oxide. Results of both experiments showed that foliar application of ZnO in mixture level in the field and nano ZnO alone in the pot experiment significantly increased most biochemical and yield traits in both barley cultivars. In both experiments, the amount of photosynthetic pigments, content of elements in plant tissues and seeds, as well as yield and yield components were higher in Julgeh inoculation with G. mosseae than in Yousef cultivar, whereas Yousef cultivar had higher superiority in tiller number per m2, plant height and biological yield while inoculated with G. intraradices. Evaluation of root traits in pot experiment showed that Yousef was superior while inoculated with G. interaradices in total root length, root length and root dry weight, whereas Julgeh showed more potential to increase root diameter and volume in inoculation with G. mosseae.