Abstarct: In this research macrocyclic Schiff baxse ligand were provided from the reaction of 1,2-bis(2-aminothiophenyl)ethane with o-phthalaldehyde in ethanol solution, then complexes of these ligand were synthesized with ions Mn(II), Fe(III),Co(II) and Ni(II). The macrocyclic Schiff baxse ligand and its complexes were characterized on the basis of the results of the molar conductance and spectroscopic studied such as IR, 1H & 13C-NMR and Mass. These ligand as tetra dentate containing atoms (N, S) coordinate to the transition mextal ions and form stable complexes. The biological activity of macrocyclic Schiff baxse ligand and their complexes against various fungi and bacteria was assessed.