Abstarct: In recent years, with the increase in life expectancy and the aging population worldwide, musculoskeletal injuries such as fractures, back pain, spinal deformities, osteoporosis, infections or bone tumors, congenital defects, and injuries to the mouth and jaw, as well as rheumatic diseases like osteoarthritis, are rapidly on the rise. Therefore, the development of bone-related treatments and the reduction of associated medical costs have attracted the attention of many researchers. This study aimed to produce a nanocomposite with a composition similar to that of bone tissue and to investigate its physicochemical and biological properties in order to find a suitable alternative for bone tissue. In this research, several nanocomposite samples with varying percentages of hydroxyapatite ceramic particles and bioactive glass in a gelatin polymer matrix were produced and examined. Among them, samples that showed greater bioactivity were selected for further testing. After adding silver nanoparticles, the samples underwent cytotoxicity testing (MTT) and antibacterial testing. The produced compounds were characterized using Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), grazing incidence X-ray diffraction (GI-XRD), flame photometry, field emission scanning electron microscopy (FESEM), and energy-dispersive X-ray spectroscopy (EDS). The results indicated antibacterial properties and non-toxicity in the samples. The samples were placed in a simulated body fluid (SBF) for 21 days to conduct bioactivity tests. Observations from SEM images and EDX tests indicated changes in the surface structure of the composite samples and the formation of calcium phosphate deposits. The FTIR analysis performed on the surface of the samples also showed an increase in the intensity of peaks related to P-O and a decrease in the intensity of peaks related to the substrate, confirming the presence of a calcium phosphate laxyer on the surface.