Abstarct: Bioactive glasses are directly used for biomedical applications as orthopedic implants and bone building materials due to their biocompatibility and strong bonding with bone. In this research, two types of bioactive glass (CaSiO3 - MgCaSi2O6) were synthesized by the sol-gel method, using ammonia as a gelling agent. It was calcined at 700, 1000 and 1200°C. Then it was added to the polymer matrix in the form of amorphous glass and glass-ceramics after heat treatment at 1000 °C. The synthesized compounds were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM), field emission scanning electron microscopy (FESEM) and Energy-dispersive X-ray spectroscopy (EDS). The results showed the synthesis of wollastonite and diopside nanoparticles with high purity. The samples were placed in Simulated Body fluid Solution (SBF) to perform the bioactivity test. the bioactivity of wollastonite and diopside nanocomposite samples with different amounts (5, 10 and 15 wt%) of the target glass with polycaprolactone matrix was investigated, and the results showed that increasing the amount of glass increased the bioactivity of the composite samples. Amorphous wollastonite was also shown to be more bioactive compared to calcined wollastonite at 1000°C, while for diopside glass-ceramic, the results show more bioactivity compared to primary diopside. Immersion in the simulated body solution and checking the pH changes show the formation of a calcium phosphate laxyer on the samples.