Abstarct: According to natural bone structure, fabrication of polymer-ceramic nano-composite scaffolds has attracted a great deal of attention as an alternative for bone grafts. Recently, polycaprolactone has extensively been used for its great biocompatibility and biodegradation, as well as hydroxyapatite nano particles as the main mineral component of bone for bone tissue engineering. Also antibacterial properties of silver particles are well known. In this research, polycaprolactone, nano-hydroxyapatite (0%wt, 15%wt, 20%wt) and silver oxide (0%wt, 3%wt, 6%wt) composite scaffolds with different compositions were prepared by freeze drying method and their properties were investigated. Scanning electron microscopy (SEM), X-ray diffraction analysis, Fourier transform infrared spectroscopy (FTIR) and mechanical test were carried out to evaluate the surface morphology, phase content, chemical bonds and functional groups and compressive strength, respectively. In addition, in vitro bioactivity of scaffolds were evaluated by soaking them in SBF for 14 days to observe apatite laxyer formation. Results demonstrated that freeze dried scaffolds had interconnected pores with suitable mean pore size (114-135 µm) for cell adhesion and proliferation. HA and silver oxide particles were well disperse in polymer matrix, and increasing in weigth percent of them, enhanced the compressive strength, as well as decreasing the porosity from %81 for pure PCL to %73 for scaffold contain %20HA/ %6Ag2O. Formation of apatite laxyer confirmed the bioactivity of the scaffolds and had direct relationship with nano-hydroxyapatite weigth percent. Finally PCL/n-HA/Ag2O composite scaffold is suitable for bone tissue engineering.