Abstarct: The most important minerals and toxic contaminants are heavy mextal ions of lead and zinc, which have been introduced into the environment due to the introduction of mineral activity, resulting in severe poisoning in humans and other living organisms. In this research, examples of contaminated soils of coal mine (Dehmola) and bauxite mine (Jajarm) have been prepared. In the next step, the bacterial species found in these soils were identified and cultured. The results show that there are 7 types of aerobic bacteria including Microbacterium SP, Planomicrobium SP, Thalassospira SP, Halomonas SP, Enterobacter SP, Brevndimonas aurantiaca and Micrococcaceae bacterium in these soils. Therefore, the potential of these 7 bacteria was investigated to remove heavy mextal ions from wastewater in single and double systems. The initial results indicate that lead and zinc ions are eliminated by Thalassospira SP and Enterobacter SP bacteria, respectively. Optimization of operating parameters including pH, initial concentration of contaminant, reaction time and temperature had done, and results showed that in the single system of lead and zinc elements at pH = 6, the initial concentration of the pollutant = 100 mg / l, the concentration of bacteria = 2 cc, time = 72 hours and temperature = 30 °C has a removal efficiency of 91.6 and 89.85%. The results in the binery system shows that the bacteria act selectively, and the Thalassospira SP bacterium only absorbs lead ion and Enterobacter SP bacteria only absorbs zinc ion, and in the presence of a suitable bacterium, second ion has little effect on the absorption of the first ion. The results of the mechanism of the process show that both bacteria can remove lead and zinc ions through a biologically and selective regenerative process.