Abstarct: Nowadays, Mineral deposits characterized by outcropping hard-rock mineralization are well explored and currently being depleted. Consequently, mineral exploration is increasingly focussing on areas where thick laxyers of overburden conceal potential. Success of explorational techniques in these areas is an understanding of processes that lead to element dispersion and concentration in the surface soils. Deposit overburdens rich in elements which are mainly used as a micronutrient for microbes. Therefore, Microbes by using these elements can flourish and can be put special biological effects on the soil. These microbial processes, causing the formation of secondary enrichment zone and microbial haloes in deposit overburden. exploration geomicrobiology, with an identification of biogeochemical processes and Recognition of structures of microbial communities of deposit overburden and other geomicrobiological processes, Sets out the concepts such as concentration, distribution, migration and transportion of mextallic elements such as, Zn, Au, Cu, Co, Mo, Fe, ... and by molecular tools and laboratory culture traditional techniques, trying to explain the geochemical and geological processes are associated with these elements. The exploration geomicrobiology consists of culture-independent techniques and Culture-dependent techniques. In this project, samples took of the four copper mine such as, Sungun, Jaru, Aliabad and Darezershk geomicrobiologically. And microbial tests including laboratory techniques and molecular methods were performed On the samples. Simply put, in the current pattern, with the choice of copper-bearing selective medium and antibiotics injection into the medium, the differences between mineralized and non-mineralized areas were identified and consequently, With bioinformatics analysis, primer design and optimization of PCR, copper resistant bacteria and the copper reserves probable bioindicator identified and introduced.