Abstarct: Neyshabour Turquoise Mine is located in the Khorasan Razavi Province and in 53 Kilometer of Northwest of the Neyshabour city, near the Binaloud mountains. There were two villages near the mine. Drinking water of two villages is provided from a spring inside of an abandoned tunnel. Due to the mining operation, local drinking waters were affected by a number of elements such as Pb, Zn, U, Cu and Cr. In order to investigate the effect of mining activity on water quality, six samples were collected from both water and sediments of the waterways in two different time period (December 2010 and January 2011). Sediments and drinking water samples were sent to laboratory for XRF and ICP-MS respectively to measure anions, cations and heavy mextal concentrations. Analysis results of sediment samples show high concentrations for Pb, U, Cu and Cr. Contamination factor (CF) calculated for Pb, Cr, U and Cu were 6.52, 1.99, 1.53 and 1.55 respectively. The CF values for these elements were high. In addition, Pollution Load Index (PLI) was computed for Pb (2.31), Cu (1.39), Cr (1.33) and Zn (1.31) that were evaluated to be high. Enrichment factor (EF) for Pb varies between intermediate and high and Muller factor was slightly high for this element. HEI and Cd indices which were calculated for all water samples were above 1 in dry season. Moreover, HPI was above 100 and HPI index was above 100 for all water samples in wet season. The types of water samples taken from spring and Qanat in both seasons were Na- bicarbonate and Na-sulfate respectively. It was found that TDS, TH, Na and SO4 of water samples measured in two seasons were slightly higher than those recommended concentrations by WHO and EPA standards.