Abstarct: Recent advances in environmental biotechnology have enabled the development of microbial bioremediation strategies for the removal of hazardous pollutants. In this study, the bioremediation potential of Microbacterium oxydans strains H3 and H7 was investigated for the biological removal of lead (Pb) and cadmium (Cd) from contaminated environments, along with the optimization of conditions influencing this process. Experiments were conducted in LB medium under controlled conditions, including fixed mextal concentrations (100 ppm Pb and 25 ppm Cd), incubation temperature of 30 °C, pH values ranging from 3 to 7, and exposure times of 5 to 25 days. The bacterial strains exhibited high efficiency in reducing mextal concentrations under specific conditions. Data were analyzed and modeled using Design Expert software. The results indicated that both strains showed significant performance in heavy mextal removal. Specifically, strain H3 achieved approximately 75.44% Cd removal under optimal conditions (pH = 6, contact time = 10 days), while strain H7 reached about 72.93% under the same conditions. For Pb, strain H7 achieved 72.98% removal under optimal conditions (pH = 4, contact time = 10 days), whereas strain H3 also demonstrated high efficiency with 80.75% removal. Statistical modeling using Response Surface Methodology (RSM) revealed that pH and contact time were the most influential factors affecting bioremediation efficiency. Overall, the findings suggest that bacterial bioremediation can serve as a sustainable, cost-effective, and efficient approach to improving the quality of heavy mextal-contaminated soils.