Abstarct: Cancer is a genetic-baxsed disease. By occurring genetic mutation, the sequence of organic baxses in genetic codes changed and. Considering that no definitive treatment for this disease has yet been found, further research and further studies from different perspectives can provide more comprehensive information in the diagnosis and treatment process, leading to faster and more accurate diagnosis. In this study, we will investigate the effect of genetic mutations on several electronic and molecular features. We used Huckel theory to model the molecular structure of DNA. According to this theory, for different parts of the DNA we created the Huckel matrices. Using the Eispack library of the Fortran software, we obtained energy eigenvalues for each of the Huckel matrices. Then, by coding in Fortran and Matlab software, we created the features as dispersion sketch, density of state, group velocity and effective mass. eventually, using these features, we investigated HOMO and LUMO energy levels, energy gap and changes in these properties in some normal and mutated sequences. From these studies, we concluded that the change and displacement in a sequence of baxses lead to significant changes in energy level and other features. the baxses sequence of the genetic code in amino acid glutamine is CAG, we obtained HOMO energy level is equal to 1.3627 ev, whereas, if the sequence changes to CCG, the HOMO energy level is changed to 1.3935 ev. Therefore, such changes can be used to diagnose and differentiate between the structure of DNA that is mutated and the structure that is not mutated and is healthy.