Abstarct:   One field of study in microfluidics is the control, trapping, and separation of microparticles suspended in fluid. Some of its applications are related to cell handling, virus detection, etc. One of the new methods in this field is using ICEK phenomena and dielectrophoresis forces. In the present study, considering the ICEK phenomena, the microparticles inside the fluid are deviated in the desired ratio using a novel ICEK microchip. The deviation is such that after the microparticles reach the floating electrode, they are trapped in the ICEK flow vortex and deviated through a secondary channel that was placed crosswise and non-coplanar above the main channel. For simulation verification, an experimental test is done. The method used for making two non-coplanar channels and separating the particles in the desired ratio with a simple ICEK microchip is an innovation of the present study. Also, the adjustment of the percentage of separation of microparticles by adjusting the parameters of the applied voltage and fluid inlet velocity is one of the other innovations of the present experimental study. We observed that for input velocities (150-1200) (µm)/s, respectively, with applied voltages (10-33) V, 100% of the particles can be directed towards the secondary-channel. In next step, using the combination of the ICEK phenomenon, and a non-coplanar and intersecting channel that has a change in cross-section, microparticles accumulate. When microparticles enter the microchannel at velocities above 1.5 m/s, after reaching the floating electrode, the microparticles are directed to a secondary channel by ICEK vortices. Microparticles accumulate in the vortices created by changing the cross-section in the secondary channel. The accumulation percentage of microparticles can be increased by adjusting the applied voltage to the electrodes around the floating electrode (driver electrodes) and the fluid inlet velocity. At a constant inlet velocity, with the increase of the electric field applied to the floating electrode, the percentage of accumulation of microparticles in the vortices of the secondary channel increases. According to the simulation and experimental results, for the input velocity of 8.5 m/s and the applied voltage of 20 V to the driver electrodes, 29% of microparticles accumulate in the secondary channel. The method used for making two non-coplanar channels, the accumulation of microparticles with a velocity above 1.5 m/s, and adjusting the number of microparticles accumulated by using the effect of the ICEK, are innovations in the present study. To validate the simulation results, a microchip was built, and then the results were compared with the numerical results.