Abstarct: Today, energy supply is one of the most important human problems. For this reason, the investigation of alternative fuels of oil and gas is extremely important. One of the alternative fuels for oil and gas are biodiesels. Biodiesels are mono-alcohol esters produced from vegetable oils or animal fats that can be used as fuel in diesel engines and burners. On the other hand, ethanol fuel is an oxygenated compound that can be ignited in burners and diesel engines. Of course, pure ethanol cannot be used in burners and diesel engines, but different percentages of it are mixed with diesel. Ethanol cannot be indissoluble in diesel and can be mixed with diesel only through an intermediary such as biodiesel. Therefore, the study of this matter is of great importance. In the upcoming research, the combination of diesel-biodiesel produced from canola oil-ethanol and the effect of changing its concentration on flame properties and emission of flame pollutants in the burner were investigated in a laboratory manner. The temperature of different flame points in the combustion chamber was measured by thermocouple. The thermal spectrum of the flame was photographed with the help of a thermal camera. Also, another imaging of the flame was done to qualitatively determine the concentration of soot particles. Combustion products were also examined with the help of a smoke analyzer. The results show that by adding biodiesel produced from canola oil up to ten percent by volume, the flame properties improve, CO decreases, CO2 and NOX increase; But by increasing the percentage of biodiesel from ten to twenty percent, the trend reverses. It is also observed that the flame properties deteriorate with the increase of ethanol percentage. This means that the temperature during the flame, radiant energy and brightness are reduced and combustion is decreasing. It is also observed in the smoke analysis that CO increases and CO2 decreases with the increase in ethanol percentage. It is also observed that NOX is reduced by adding ethanol, which is one of the benefits of adding ethanol to fuel.