Abstarct: Abstract: Due to the growing demand for energy, it is necessary to make optimal use of renewable energy sources. Extraction of energy by anaerobic digesters from biomass as a renewable source in addition to producing methane gas is also a suitable method for the treatment of perishable organic matter, which greatly reduces the pollution load of organic waste in the household, agriculture, livestock and wastewater will be. In this research, anaerobic digestion was designed and constructed with the ability to use different types of biomass. Due to the importance of digestion and the need for research in the laboratory, in this study, first, the types of anaerobic digestion were monitored and the cases that improve the speed and quality of biogas production were investigated. Then, laboratory anaerobic digestion was performed and its outputs were analyzed using different types of biomass. Biomass of Holstein dairy cattle manure and combined biomass of dairy manure and horse manure as well as the effects of mixing and concentration of the mentioned biomass were studied and also the effects of temperature, concentration and mixing in digestion in thermophilic environment of methane gas were investigated. The results showed that cases such as stirring in 4-hour periods for 5 minutes and combining horse manure with cow manure in a ratio of 1 to 3 due to reduced acidification time increased the percentage of methane from 58 to 62% in biogas produced. Also, the appropriate concentration of substrate in the thermophilic environment is between 10-7% of the solids and more than ten percent of the solids in anaerobic digestion in the thermophilic environment does interfere with the process and not enter the methane generation phase.