Abstarct: To separate the particles in a microfluidic device, a magnetic field can be used along with sputtered magnetic electrodes at the bottom of the microchannel. The proposed design consists of a microchannel with a length of 30 mm with three different outlets. Sputtering of several nickel electrodes (with different angles and thicknesses) at the bottom of microchannel was need for separation of diferent particles. In order to validate the results, numerical solution was compared with analytical relations. Important parameters such as different microparticle sizes, wire dimensions, wire spacing, wire angle and the flow rate were analyzed. The results show that M-450 particles are affected by stranger force in comparison with Myone and oligo dT (25) microparticles due to their higher magnetism and size. It was shown that by increasing the angle of the wires (at the bottom of the channel), the particle deviation was reduced. Also, three thicknesses of 10, 15 and 20 microns were investigated for sputtered electrodes. The maximum deviation was related to the thickness of 20μm, so that the particles were not able to pass over the electrodes. By increasing the distance between the wires (from 400 μm to 700 μm), the microparticle deviation was increased. As the flow rate of the inlet fluid increases (from 25 to 75 mm / s), the hydrodynamic force on the microparticles increases and therefore, the particle deviation decreases while passing over the electrodes. It was observed that for speed of 50 mm / s, the best sparation efficiency (94%) was obtained for the proposed microchip.