Abstarct: Fossil fuels have been a source of energy in internal combustion engines. The increasing use of fossil fuels, increasing the petroleum products and decreasing the available reserves have encouraged researchers to find new energy sources. On the other hand, productions of environmental pollutants including Unburned Hydrocarbons (UHC), Nitrogen Oxide (NOX), and Carbon Monoxide (CO) are among the main disadvantages of fossil fuels. In order to conserve the available resources and reduce the environmental pollutants, the tendency to new energies and alternative fuels are considered as an obvious phenomenon. Biodiesel is among the renewable fuels. Biodiesel is ethylester or methylester that is produced from vegetable oils or animal fats and is used as fuel in diesel engines or heating systems. Three main methods that have been applied in order to replace vegetable oils consist of pyrolysis, micro-emulsification, and transesterification. In this study, transesterification process was used to produce biodiesel. This choice was baxsed on three factors including production simplicity, low needed tools and high efficiency. In this method, methanol (as alcohol) with the ratio of 6/1 (alcohol to oil) and sodium hydroxide (as catalyst) with the ratio of 1% oil weight were used. The transesterification process of biodiesel production was performed using three methods of mechanical stirring, ultrasonic homogenizer and hydrodynamic cavitation. Therefore, the optimal conditions for biodiesel production were determined baxsed on these three methods. In mechanical stirring method, the temperature was examined in the levels of 35, 50 and 65°C. Also, the stirring intensity was investigated in three levels of 350, 500 and 650 RPM. In ultrasonic homogenizer method, the power of device was examined in three levels of 100, 150 and 200 W. Also, the pulse frequency was examined in three levels of 0.4, 0.7 and 1.0 during 15 minutes. In hydrodynamic cavitation method, Grooved Venturi and Perforated Plate were used and the production conditions of biodiesel fuel in four internal levels of 2, 3, 4 and 5 bar were investigated during 60 minutes.