Abstarct: Microfluidic systems have extensive applications in the fields of medicine and industry. Their main applications include disease diagnosis, drug delivery, gene therapy and thermal therapy. These systems can be used for various purposes such as collecting, separating, mixing, sorting and concentrating micro-particles or cells. In this research, a magnetophoretic-baxsed microfluidic system which is presented, enables the manipulation of magnetic particles within a microchannel. Since magnetic force is required to direct the particles in magnetophoresis and this force arises from the magnetic field gradient, a novel design for generating a magnetic field gradient with precise control over its intensity has been developed in this system. For the purpose of generating a magnetic field, an electrode is placed at the bottom of a channel with an electric current passes through it. By creating a cavity within this electrode, the cross-sectional area through which the electric current flows is altered, causing a localized variation in the current-to-surface ratio. This modification leads to the creation of a localized gradient within the microchannel. Using this system, a magnetic particle sample can be separated from other non-magnetic particles within the carrier fluid, or two magnetic particle samples with different properties (such as size or magnetic permeability) can be distinguished from each other. Since the electrical current intensity in this design is controllable, the system can be used for cells and particles of various sizes and flow rates, allowing them to be transported and manipulated to desired locations as needed. In this research, the proposed system is first analyzed through numerical simulation, and a laboratory model is constructed baxsed on the numerical results. During the numerical analysis, the system parameters are identified, and using COMSOL Multiphysics software, the effect of these parameters on the displacement of three magnetic micro-particle models M-280, M-450, and magnetite (Fe₃O₄) is examined.