Abstarct: Antibiotics have several negative effects on aquatic ecosystems and are difficult to degrade using traditional water/wastewater treatment methods. As a result, new treatment techniques must be employed to eliminate these contaminants from aquatic environments. Research on the relationship between the decomposing process of antibiotics and different factors by new technologies is scarce. This research focuses on the capability of ozone micro-nano bubbles (OzMNBs) to eliminate the Fluoroquinolone antibiotics (ciprofloxacin (CIPR), levofloxacin (LEVO), ofloxacin (OFLO) and enrofloxacin (ENRO)) in aqueous solutions. We studied the CIPR, LEVO, OFLO and ENRO decomposition to different variables through the central composite design method. The main variables included pH, ozonation time, and initial antibiotic concentration. The correlation coefficients of the quadratic model obtained by using the software, Design Expert version 13.0.1. Analysis of variances proved the significance of models and main factors. Verification tests also confirmed that the final optimum conditions of the antibiotics decomposition were: pH 9, ozonation for 40 min and, initial antibiotic concentration of 5 mg/L. The order of influence of various factors on Fluoroquinolone antibiotics decomposition were obtained and the interactions between the main factors were also investigated. Furthermore, the influence of the OzMNBs on the toxicity of the sampes containing antibiotics and the efficiency of OzMNBs in the removal of total organic carbon and mineralization of the solutions under optimum conditions were investigated. At the last stage of the research, the decomposition mechanisms of the antibiotics were examined.