Abstarct: This study examines the effects of mycorrhizal fungi, Thiobacillus bacteria and sulfur fertilizer on quantitative and qualitative properties of garlic in the field of agriculture and natural resources university of sari in 1390 in a randomized complete block design with three replications. In this study, each replication consists of 12 plots. Treatments include mycorrhizal inoculation( M, )Thiobacillus( T,)M+T75 g sulfur per hectare (75S,) 75S + M, 75S + T, 75S + M + T, 150kg of sulfur perhectare( 150S,)150S + M, 150S + T, 150S + M + T. The results showed that the application of 150 kg of sulfur per hectare treated with Thiobacillus and mycorrhiza significantly affected on yield. Treatment combination of 75 and 150kg of sulfur perhectare with mycorrhiza, increased 31and 68 percentyield respectivelycompared to control. Inoculation with mycorrhizal fungi and 150 kg sulfur perhectare were significantly increased phosphorus percentage of soil. The results of thisstudy showed that Maximumroot colonization observed with mycorrhizal application, so that root colonization was observed about 70%. The results of this research revealed that the addition of sulfur and Thiobacillus bacteria increased soil sulfur about 122% compared to control. Thiobacillus and sulfur application led to a significant decrease in the percentage of root colonization. Inoculated with Thiobacillus bacteria associated with 75 and 150 kg of sulfur perhectare on plant height, dry yield of bulbs, number of clove, bulb diameter, chlorophyll a, carotenoids, essential oil yield, phosphorus and sulfur amounts of soils, dry weights of clove, root, stem, scape and leaf and also bulb fresh weight were statistically significant. Overall, these results suggest that aplication of sulfur and Thiobacillus bacteria and mycorrhizal inoculation had positive effect on growth, yield and quality characteristics of the garlic plants.