Abstarct: Wheat is an important cereal to be used as staple food and feed. Fusarium head blight (FHB, scab), caused by Fusarium graminearum,is a major disease, affecting wheat and other cereals particularly in places with relatively semi- to high humidity. FHB damages plants at heading stage, causing a large economic loss. The fungus produces a mycotoxin known as deoxynivalenol (DON) that affects grain yield and quality. Trichothecene mycotoxins (¬such as DON¬) are potent protein synthesis inhibitor with harmful effects on human and livestock. Chemical modification of DON could reduce its accumulation in grain. AYT1 encodes an enzyme that transfers an acetyl moiety to the C3 hydroxil group of trichothecene. in this study, Second-generation of seeds with AYT1 were planted and the seedling were tested for the presence of the gene via PCR and RT-PCR. Transgene copy number was established via Southern analysis that was indicative of having more than one copy number within the three transgenes tested. Moreover, thin laxyer chromatography (TLC) was showed the presence of the encoded AYT1 protein. Additionally, bioassay results demonstrated that treatment with the fungal pathogen causes DON detoxification and partial transgenic plant protection