S751 : The effects of plant growth regulators on somatic embryogenesis in vitro culture of salvia leriifolia Benth
Thesis > Central Library of Shahrood University > Agricultural Engineering > MSc > 2019
Authors:
[Author], [Supervisor], [Advisor]
Abstarct: Abstract Norozak (Salvia leriifolia) is a medicinal plant in danger of extinction. Therefore, the effect of different concentrations of plant growth regulators and explants on indirect somatic embryogenesis for in vitro micro propagation was investigated. In the callus induction phase using embryo, cotyledon, root, stem and leaf explants and application of 0, 0.2, 0.5, 1, 2 and 3 mg/l 2,4-D showed that the highest percentages of callus induction (100%) in root explants at 0.2 mg/l 2,4-D, maximum callus diameter (6.031 mm) in cotyledons at 0.2 mg / l concentration, highest weight (0.706 gr ) in leaf at0.2 mg / l concentration and maximum dry weight (0.123 gr ) were observed in fetal explants at 0.5 mg / l. At the somatic embryogenesis induction stage and development (globular to cotyledon), a combination of four plant growth regulators was used. The duration of differentiation stages and the number of obtained plantlets from somatic embryogenesis were indicated the highest number of plantlets (5) was observed in root explants on medium supplemented with 2 mg/l BAP + 0.5 mg/l KIN + 1 mg/l NAA and the lowest embryo development time was 48 days in leaf explants on medium supplemented with 0.5 mg/l 2,4-D + 2 mg/l BAP . Microscopic photographs of the explants confirmed the embryogenesis.
Keywords:
#Key words: Salvia lerrifolia B. #Plant growth regulators #explant #Somatic embryogenensis #Histology. Keeping place: Central Library of Shahrood University
Visitor: