Abstarct: In order to study the effect of some organic and bio-fertilizers on and yield of tomato and their impact on the analysis of metribuzine residue in soil, a Factorial experiment was conducted in a randomized complete block design with three replications at the Faculty of Agriculture, Shahrood University in 2011. Threatments included three levels of organic fertilizers (vermicompost, cow manure, and control) and biological fertilizer in four levels (Pseudomonas putyda, Pseudomonas fluorescens, Azotobacter chrococcum and control). In addition to measurements of traits and yield of tomatoes, for determination of metribuzine residue, soil sampling was taken from 0 to 15 Cm soil depth, (2 hours), 3, 8, 32, 55 and 90 days after application.The results showed that vermicompost manure and Azotobacter chroococcum increased the total number of fruits 42 and 37 percent, stem dry weight 22 and 24 percent and total weight of tomato fruits 13 and 17 percent respectively compared to the control treatment. The results showed that chlorophyll content, leaf dry weight and stem diameter was increased 23, 53 and 46 percent respectively by application of vermicompost with Azotobacter chroococcum. The interaction bitween cow manure × Azotobacter chroococcum and vermicompost × Pseudomonas putida, increased relative water content and the number of green fruit 28 and 46 percent respectively compared to the control. The bacteria population was increased 86 percent by the interaction effects of vermicompost × Pseudomonas fluorescens. The results showed that the lowest extraction performance of metribuzine (73 /94percent) and the highest of it (95/12 percent) were related to vermicompost and cow manure treatment respectively. The lowest rate of decay, half-life and 90- percent decay point was related to application of cow manure treatment with Azotobacter chroococcum. While, the highest levels of it was found in combination of vermicompost and Pseudomonas fluorescens treatments. Thus, the treatment cow manure with Azotobacter chroococcum reduced 23 percent rate of decay, half-life increased by 26 percent and increased 90 percent compared to 26 percent degradation was observed. Thus, application of vermicompost with Pseudomonas fluorescens increased the rate of decay by 37 percent, and decreased the half-life of herbicide 52 percent and 90- percent decay point 54 percent than control treatment. baxsed on our results, organic and biological fertilizers by their Synergistic effects in addition to increasing yield of tomato can change the rate of metribuzine decay and persistence in soil.