Abstarct: Like other members of the Brassicaceae family, there are many sulfur compounds in the vegetative organs and seeds of rapeseed, and it has been proven that sulfur has a direct effect on the quality, growth and efficiency of the seeds of this plant. The presence of sulfur compounds as an important factor has a significant effect on the optimal seed yield in all types and cultivars of canola, and the use of sulfur has been very necessary. To supply the sulfur required by this plant, various sources and methods can be used, which of course must be tested. For this purpose, an experiment was conducted in Shahrood University of Technology in 1401 to investigate the effect of foliar spraying and seed pretreatment with different sulfur-containing compounds on the growth, yield and physiological traits of spring rapeseed. Factorial experiment baxsed on a randomized complete block design with three replications and treatments including foliar spraying of compounds containing sulfur at three levels (zero, liquid sulfur and sodium hydrosulfide) and seed pretreatment with compounds containing sulfur at six levels (zero) , distilled water, liquid sulfur, sodium hydrosulfide, thiamin and biotin), which was applied on the spring rapeseed plant of Hyola 401 variety. Liquid sulfur with a concentration of 1.5 per thousand and sodium hydrosulfide as a hydrogen sulfide releaser with a concentration of 0.6 mM were sprayed before the flowering of the plant. The concentration of 100 mg/liter was used for pretreatment of thiamine and biotin, and the same concentration of foliar spraying was used for pretreatment of liquid sulfur and sodium hydrosulfide. According to the results obtained from this research, foliar spraying and seed pretreatment with sulfur-containing compounds improved most of the tested traits. Although, in some traits, conditions without foliar spraying and pre-treatment were relatively superior. Spraying with sulfur-containing compounds increases vegetative traits (plant dry weight, stem diameter and number of sub-branches), physiological traits (relative content of leaf water, anthocyanins and flavonoids), yield traits (seeds in capsule and seed viability) and qualitative traits. (oil percentage and seed oil and protein yield). On the other hand, seed pre-treatment with sulfur-containing compounds, except for liquid sulfur, increased all tested traits. In non-sprayed plants, thiamine pretreatment with 466.2 gr/m2 had the highest seed yield, which showed a 68% increase compared to the control. With the application of sodium hydrosulfide foliar application, the highest percentage of oil was related to sodium hydrosulfide, biotin and thiamine pretreatments with the amounts of 43.93, 43.3 and 42.96%, respectively. In all three conditions without foliar spraying, foliar spraying with liquid sulfur and sodium hydrosulfide, the highest percentage of protein was related to pretreatment with thiamine with the amount of 19.96, 20.2 and 19.56%. Finally, in this research, foliar spraying with liquid sulfur and seed pretreatment with thiamine were chosen as the superior treatment combination due to the increase of most of the measured traits.