S630 : Investigation of agronomical and physiological responses of green beans (Phaseoulus vulgaris) to nanosilver heavy mextal and foliar application of citric acid and hydrogen sulfide
Thesis > Central Library of Shahrood University > Agricultural Engineering > PhD > 2021
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Silver nanoparticles are being extensively used in a broad range of applications in our daily routine life. In the present study, it was investigated if citric acid (CA) and hydrogen sulfide (H2S) can mitigate adverse effects of silver nanoparticles (AgNPs) in green bean plants. Green bean seedlings grown in various conditions (in vitro, greenhouse and field) were applied with AgNPs either through soil drenching or foliar spray and were then treated with different concentrations of citric acid and NaHS, as H2S donor. Germination assay experiments were performed in three stages in a completely randomized design with three replications. Green bean seeds were treated with nanosilver solution with various concentrations (0, 0.5, 1, 1.5, 2, 2.5 g per liter and distilled water), citric acid (0, 0.5, 1, 1.5, 2, 2.5 g/l and distilled water) and sodium hydrosulfide as hydrogen sulfide donor (0, 0.3, 0.6, 0.9, 1.2, 1.5 mM and distilled water) in three-time courses for 5, 10 and 15 hours. Greenhouse and field experiments were performed in as factorial in a randomized block design. Greenhouse treatments including nanosilver treatment at four levels (control, foliar application with a concentration of 2.5 g/l, soil application with a concentration of 2.5 g/l and combined application of foliar application and soil with the mentioned concentrations), citric acid spray at three levels (0, 0.75 and 1.5 g/l) and of sodium hydrosulfide spray as a donor of hydrogen sulfide at two levels were the conducted (control and 0.6 mmol) .The treatments in the field included nanosilver foliar application at three levels (0, 1.5 and 2.5 g/l), and citric acid and sodium hydrosulfide foliar application were similar to the pot experiment. The results of in vitro experiments showed that seed pretreatment with all three combinations of nanosilver, hydrochloric acid and hydrogen sulfide improved germination parameters and seedling growth, meanwhile, the effect of nanosilver pretreatment was more than the other two treatments. Although in some traits, hydroprime and control level showed relative superiority. In greenhouse and field experiments, the application of nano-silver had a negative effect on the vegetative traits including root length and volume, total biomass and physiological traits such as chlorophyll a, b and carotenoids of green bean plants. and its negative effects were higher in the greenhouse with the combined use of soil application + foliar application and in the field stage at a concentration of 2.5 g/l. Nano-silver stress increased the percentage of electrolyte leakage, the amount of hydrogen peroxide and the activity of antioxidant enzymes. Foliar application of citric acid and sodium hydrosulfide improved vegetative traits (shoot dry weight, number of branches, number of pods per plant and green yield) and photosynthetic pigments. The application of these two treatments reduced the activity of antioxidant enzymes and the amount of electrolyte leakage through mitigating the effects of stress, and also improved the relative content of leaf water and soluble protein. Concentrations of 1.5 g/l citric acid and 0.6 mM hydrogen sulfide were the best treatment composition in both greenhouse and field conditions was, which made the plants more resistant against all nanosilver treatments.
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#Keywords: Antioxidant enzymes #Stress #Germination #Photosynthetic pigments #Sodium hydrosulfide Keeping place: Central Library of Shahrood University
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