S577 : Identification of Cyclamen species in the South East of the Caspian Sea and Comparison with indigenous Turkish Cyclamen
Thesis > Central Library of Shahrood University > Agricultural Engineering > MSc > 2021
Authors:
Abstarct: In the present study, to evaluate the morphological diversity of wild cyclamen genotypes in the south east of Caspian Sea and comparison with indigenous Turkish Cyclamen and identify the species of Iranian genotypes, 400 Iranian cyclamen genotypes with 200 turkish were studied.The genotypes were collected from four regions in Iran including Hezar Jerib, Glidagh, Alangdere, and Garmabdasht, as well as tree regions in Turkey including Adana, Osmaniye, and Izmir. Generally, 27 quantitative and qualitative traits were evaluated using a cyclamen descxriptor. The results of principal component analyses (PCA) of Iranian genotypes showed that the highest and the lowest coefficient of diversity were related to the number of flowers and the length of the petals, and the length of the female, respectively. In Turkish genotypes, the highest and the lowest coefficient of diversity were related to the darkness of the petal margin and the width of the petals, respectively. Comparing the genotypes of the two countries, the highest and the lowest coefficient of diversity were related to the dark margin of the petals and the design on the leaves, respectively. PCA results of Iranian genotypes showed that the first three factors explained 61.41% of the variance of the total data. The most effective traits in this analysis in the first component were the basal corolla ring color, color of upper corolla, color of lower corolla, darker petal margin, stigma position and leaf pattern color. PCA results of Turkish genotypes, showed that the first three factors explained 82/56% of the variance. The most effective traits in this analysis in the first component were the darker petal margin, stigma position, diameter tuber, pedicel coiling, flowering.
PCA results of Turkish and Iranian Cyclamen, showed that the first three factors explained 57% of the variance. The most important traits in this analysis in the first component were the lamina (degree of lobing) and plant growth habit. Biplot analysis was determined by two main factors of genotype distribution, which showed that Iranian genotypes of Hezar Jerib, Glidagh and Garmabdasht regions were in the same plot with Turkish genotypes in Ottoman region so, they are similar. In general, the cluster, biplot and diplate analysis lead to Iranian genotypes were very similar to the genotypes of Ottoman region of Turkey. Since the cyclamens of Ottoman region belong to C.coum species, it is possible that species of Iranian cyclamen collected is this species. In addition, very high morphological diversity was observed in wild cyclamen, which they can be used in breeding programs.
To determine the most appropriate stage of pollen grains of wild cyclamen gynotypes for pollination, the germination of pollen grains was studied. The percentage of pollen grain germination of a collected genotype of cyclamen plant from Hezar Jerib of Mazandaran province in an experiment with three
032
levels of calcium nitrate (0, 300 and 600 mg/l), three levels of boric acid (0, 200 and 400 mg / l), and three pollen harvest times (1, 3 and 6 day after flowering) showed that the highest germination percentage of pollen grains was recorded 85% and 87/6%, three days after flowering in the medium containing 300 mg/l calcium nitrate and 200 mg/l boric acid and six days after flowering in the medium containing 300 mg/l calcium nitrate and 400 mg/l boric acid, respectively. On the first day of flowering, the germination percentage was low. In general, in the breeding program of Cyclamen, it is better to use pollen grains of the third day opened flowers.
Keywords:
#Morphological diversity #Descxriptor #Breeding #Pollen grain #Calcium nitrate Keeping place: Central Library of Shahrood University
Visitor:
Visitor: