Abstarct: Drought stress is the most important factor limiting the growth and yield of medicinal plants, including Marigold. Given the water shortage in the country and its importance in the production of medicinal plants, it seems necessary to apply management methods that produce good performance with minimal irrigation. Therefore, seed pretreatment with organic matter and nanofertilizers in order to precisely control the release of seed nutrients and due to physiological and morphological changes can be an effective step towards achieving sustainable agriculture and increasing plant tolerance to stress. In order to investigate the effects of seed pretreatment on drought tolerance, growth and active ingredients of Marigold (Calendula officinali L), an experiment was conducted in the year 2016-2017 as a compound (repeated in place). This experiment was performed in two independent sections (including germination test and field test). The germination experiment was performed during two independent experiments. The first experiment was performed as a factorial in a completely randomized design with three replications. The experimental treatments were dry levels of polyethylene glycol at four levels (control, 3-, 6). And 9 times) and prime with nano fertilizers (control, Iron oxide, Zinc oxide and Boron oxide with a concentration of two per thousand). The second experiment was performed as a factorial in a completely randomized design with three replications. The experimental treatments were dry levels of polyethylene glycol at four levels (control, -3, -6 and -9bar) and priming treatments including Humic acid. (72 mg / L), Salicylic acid (2000 μmol), Ascorbic acid (200 ppm) and control were applied. The field experiment was conducted as a factorial experiment in the form of a randomized complete block design with three replications in combination (repeated in situ) located in the research farm of Urmia and Salmas Agricultural Research Center. The first factor is the method of irrigation in two levels including irrigation and cessation of irrigation at the beginning of flowering and the second factor of priming treatments in seven levels including pretreatment with nano fertilizers (Iron oxide, Zinc oxide and Boron oxide with a oncentration of two per thousand) and pretreatment with organic matter (Humic acid 72 mg / l, Salicylic acid 2000 μmol and Ascorbic acid 200 ppm) and control. Germination test results showed that with increasing levels of polyethylene glycol-induced dryness, germination indices are significantly reduced. The highest value of the measured traits was observed in normal irrigation and in priming treatments and the lowest value of the mentioned traits was observed in the -9 bar drought level due to polyethylene glycol in the control treatment. In the first germination experiment, the greatest effect under drought stress conditions was obtained on the final germination percentage, seed vigor index, root length, and seedling length due to pretreatment with iron nanoxide andThere was no significant difference in germination rate and shoot length of iron nanoxide and zinc had the greatest effect on both. The highest allometric coefficient in normal irrigation due to pretreatment with Iron nanoxide was 3.27% and the lowest in the drought level -9 bar in the control treatment (no pretreatment) was 1.96%, which was a significant difference with other priming treatments. In the second germination experiment, the interaction effect of pretreatment with organic matter and drought stress on shoot length, root length, shoot dry weight, allometric coefficient, seed vigor index, germination percentage and germination rate was significant at 1% probability level. According to the results, in general, with increasing drought stress, the measured germination parameters decreased, so that the minimum values of germination parameters were observed at the -9bar drought level . At the drought level, all priming treatments significantly increased germination percentage and germination rate compared to the control, but there was no significant difference with each other.In seed vigor index traits, seedling dry weight and stem length were the most affected by pretreatment with salicylic acid. The greatest effect on root length at all drought levels was obtained by pretreatment with Humic acid. The effect of pretreatment with salicylic acid and Humic acid on seedling length was significant at all drought levels but there was no significant difference between the two treatments. In all drought levels, the highest allometric coefficient was obtained by priming with salicylic acid, which had a significant increase compared to the control and other treatments. The results of combined analysis of field experimental data showed that the use of seed priming with nanomaterials and organic matter in traits such as plant height, number of capitols, plant dry weight, root dry weight, grain yield, harvest index, percentage of essential oil and percentage of mineral element Phosphorus showed a positive reaction and increased and improved the mentioned traits and quantitative and qualitative performance in Marigold, especially in drought stress conditions. Pretreatment with Zinc nano oxide and Iron nano oxide caused a significant increase in oil performance compared to the control and other priming treatments. The results of combined analysis of experimental data showed that under drought stress, the application of salicylic acid pretreatment caused a significant increase in proline accumulation compared to the control. Regarding antioxidant properties in drought stress conditions, application of pretreatment of Iron nanoxide, Humic acid and Salicylic acid significantly increased the activity of catalase and peroxidase enzymes compared to the control (no treatment) and other treatments. . In general, seed pretreatment with different levels of Salicylic acid and Iron and Zinc nanoxide significantly reduced the adverse effects of drought stress on most of the studied traits. Plants from seeds treated with Salicylic acid, Zinc and Iron nanoparticles had higher stress tolerance. The results of this study showed an increase in drought stress tolerance and improved Marigold yield under stress conditions by applying seed priming with Salicylic acid, Iron nanoxide and Zinc nanoxide, which is recommended in sustainable and organic agriculture.