Abstarct: Quinoa (Chenopodium quinoa Willd) which is an annual plant contains high nutritional and economically is important and consequently, the cultivation of this plant is developing in the world. Due to importance of quinoa in the arid region, in this study, the effect of seed priming on yield and some morphological and physiological trait under drought stress conditions were investigated. This research was carried out in 2019 and a factorial split plot experiment baxsed on a randomized complete block was designed with four replications in two regions namely Bastam and Damghan with different climates. In this research project the experimental treatments were included irrigation, as a main factor, at three different levels of 100, 75 and 50% water requirement (WR) and sub-factors included three varieties (Titicaca, Q26 and Q29) and finally, priming at 2 levels, (no priming and hydropriming) was implemented as factorial experiment into sub-levels. Hydropriming treatment was performed on seeds, then treated seeds were grown in the field after complete drying. To perform water requirement treatment, WR were calculated using the CROPWAT program and then applied on the plants in 6-leaf stage. To measure biochemical traits, sampling was carried out from the fully developed upper leaves during full flower stage. Also, in order to determine the grain yield and yield composition, ripe grains with relative drying were harvested from 4 square meters of each plot. The results of variance analysis were significant for most traits. Also, the results of the comparison of the mean of irrigation interactions in the variety showed that the highest concentrations of Chla, Chlb and carotenoids in all three varieties were obtained in 100% WR. Also, seed priming increased the concentration of Chla and Chlb in Titicaca and Q26 varieties, moreover, the highest activity of SOD enzyme was observed in Titicaca variety upon 75% WR whereas, the highest activity of APX and CAT enzymes was observed in all three varieties in 50% WR. The results of mean comparison showed that the priming affected grain yield in all three varieties in which the highest grain yield in Q29 variety was obtained in Bastam region with a yield of 1603.5 kg / ha. However, the highest grain yield was obtained in 100% WR and Q26 variety (with the highest leaf area index) in Bastam region with an average of 1475 kg / ha. Finally, the highest biological yield in Bastam region was obtained in Q26 variety with 100% WR and an average of 12100 kg / ha. The results of study revealed that in all three varieties, by increasing the drought stress, the percentage of seed protein increased so that the highest percentage of seed protein was obtained in Titicaca variety under 50% WR condition with an average of 19.66% in both Bastam and Damghan regions. In summary, the results showed that seed priming significantly increased grain yield, biological yield, leaf area index and chlorophyll content in cultivars in different regions under drought stress. These results indicated that the seed priming play an important role in increasing drought resistance of quinoa in low irrigation conditions.