Abstarct: In order to investigate different types of nitrogen fertilizer and cultivation pattern of corn and fenugreek mixture on quantitative and qualitative yield of forage, an experiment was conducted in two locations (Sarbisheh-Birjand and Khezri-Qa'en plain) in south Khorasan province at 2016-17. The experiment was conducted as split plot (nitrogen fertilizer as main treatment and different cultivation patterns as sub-treatment) in a randomized complete block design with four replications. Treatments included five main treatment levels (no fertilizer, 100% Urea fertilizer, 50% Urea fertilizer, Nitroxin biofertilizer, Nitroxin fertilizer + 50% chemical fertilizer) and six sub-treatment levels (pure fenugreek and corn cultivation, 100 Maize + 25% Fenugreek, 100% Maize + 50% Fenugreek, 100% Maize + 75% Fenugreek and 100% Maize + 100% Fenugreek). The results showed that the effect of nitrogen fertilizer sources and cropping pattern on corn height, 1000 kernel weight and fresh and dry weight of maize were significant in both regions. The effect of nitrogen fertilizer and cultivation pattern on fenugreek height in Qa'en was significant but not significant in Birjand region. The highest corn height was observed using 100% chemical fertilizer (195.7 cm) and the highest fenugreek height was obtained by using integrated fertilizer treatment (81 cm). The highest corn dry weight in Birjand was obtained using 100% chemical fertilizer (2056 g/m 2) and the highest corn dry weight in Qa'en was obtained by using fertilizer (3016 g/m 2). The simple effect of nitrogen fertilizer sources and cropping pattern and location, interactions of factors and their triple effect on forage quality traits were significant. The highest crude fiber content (34.41%) was observed in pure maize cultivation and the use of 100% chemical fertilizer in Birjand, the highest insoluble acid detergent fiber (39.26%) was observed in pure maize cultivation using biofertilizer in Birjand, the highest Non-soluble neutral detergent fiber (49.89%) was observed in pure maize cultivation with biofertilizer in Qa'en, highest soluble carbohydrate (33.58%) was observed in pure maize cultivation and 100% chemical fertilizer application in Birjand, highest ash (7.15%) was observed in pure culture of fenugreek with 50% chemical fertilizer in Qa'en, the highest crude protein (25.8%) was observed in pure culture of fenugreek with biological fertilizer in Qa'en, highest dry matter of frxame digestion (83.47%) was observed in pure fenugreek culture using 100% chemical fertilizer in Birjand, highest dry matter intake (4.56%) was observed in pure fenugreek cultivation using integrated fertilizer in Birjand, highest mextabolic energy (12.9 MJ), The highest specific lactation energy (2.05 Mcal/kg) and the highest relative nutritional value (284.87%) were obtained in pure fenugreek culture and 100% chemical fertilizer application in Birjand. According to the results of the experiment it can be recommended that for the proper quantity of maize and good quality of forage production, in the test areas instead of pure maize cultivation, use of maize + 75% fenugreek and maize + 100% fenugreek pattern with combined fertilizer application.