Abstarct: Although the use of chemical fertilizers may be effective on production increase, but due to decreased soil organic matter and accumulation of toxic substances in soil can cause illness amplification and loss in the product. The effect of organic fertilizers on parameters such as soil texture, water holding capacity, and reduce the acidity cause physical and chemical correction of soil and conditions improve of soil. Therefore a research has been down in order to investigate vermicompost and mycorrhiza effect and their combination on qualitative and quantitative characteristics Thymus vulgaris in the year 1390 in experimental farm of agriculture collage of shahrood. Experimental design was on the basis of randomized complete block design with 12 treatments in 3 replication. The treatment included vermicompost in 4 levels (0, 2, 4, and 6 ton.ha-1) and mycorrhiza in 3 levels (without inoculation, inoculation with Glomus mosseae and Glomus intraradices). According to the results of the interaction of vermicompost and mycorrhiza on colonization, chlorophyll a, chlorophyll b, carotenoids, total chlorophyll, essential oil content and essential oil yield were significant. Also vermicompost has had a significant effect on the height and dry weight. So that the most increase obtained with the use of 6 ton.ha-1 vermicompost and the lowest amount in control treatment. Just mycorrhiza has had a significant effect on colonization, chlorophyll b and total chlorophyll content. Thymol and carvacrol, which are major constituents in the essence, in this experiment were not affected by treatments also carvacrol in the essential oil was not made. But the main effect of vermicompost and also interaction with mycorrhiza on thymol yield was significant. Since the cultivation of medicinal plants for plant quality health of fertilizers should not be used According to the results obtained for production increases of thyme can be substituted vermicompost instead fertilizers that to be removed a step toward sustainable agriculture and environmental health.