Abstarct: In order to Study of the effect of biological fertilizer (Barvare-2), Rhizobium japonicum and seed priming on growth and yield of soybean, an experiment was carried out as factorial baxsed on Randomized complete Block Design (RCBD) with 4 replications in Agriculture Research Station of Shahrood University of Technology. Biological fertilizer including 2 levels (using of biological fertilizer and Non fertilizer) and Rhizobium japonicum including 2 levels (using of Rhizobium japonicum and Non Rhizobium japonicum) and priming including 3 levels [(hydropriming, osmopriming and control(without priming)]. Results showed that biological fertilizer significantly affected height plant, number of pod seed per plant, number of seed per plant, 1000-seed weight, grain yield, percent of protein, percent of oil and harvest index. Rhizobium japonicum significantly affected number of pod seed per plant, number of seed per plant, 1000-seed weight and grain yield, percent of protein, percent of oil and biological yield. Traits priming significantly affected all of studied traits except number of seed per pod and harvest index. Interaction between biological fertilizer and Rhizobium japonicum significantly affected number of pod per plant, number of seed per plant and percent oil. Interaction between biological fertilizer and priming significantly affected number of pod per plant.Interaction between Rhizobium japonicum and priming significantly affected height plant, number of seed per plant percent of protein and percent of oil. Interaction between biological fertilizer and Rhizobium japonicum and priming significantly affected height plant, number of seed per plant percent of protein and percent of oil. Finally, biological fertilizer and Rhizobium japonicum and priming promote growth and yield of Soybean and in addition to establishing a better environment for plant, Protecting the environment from agricultural inputs can be destructive.