Abstarct: In order to evaluate the effect of biopriming on sugarcane cuttings and legume seeds with mycorrhizal fungi and rhizobium bacteria on the yield of sugarcane (Saccharum officinarum L.), cowpea (Vigna unguiculata L.) and soybean (Glycine max L.), root dry weight, stem height and diameter of sugarcane , type of harvest index of sugarcane, cowpea and soybean, land equivalent ratio (LER), Leaf Area Index (LAI), Light Extinction Coefficient (K), weed control, Radiation Use Efficiency, water productivity, brix percentage of sugarcane, purity percentage of sugarcane, chlorophyll content in leaves in the intercropping of sugarcane and legumes, an experiment was conducted in a randomized complete block design with 14 treatments and four replications during 2016-17 at two locations in Ahwaz. The treatments were: pure sugarcane, pure soybean, pure cowpea, pure soybean + rhizobium, pure cowpea + rhizobium, pure sugarcane + mycorrhizal, Intercropping sugarcane with cowpea, intercropping sugarcane with soybean, intercropping sugarcane with cowpea + rhizobium, intercropping sugarcane with soybean + rhizobium, intercropping sugarcane + mycorrhiza and cowpea, intercropping sugarcane + mycorrhiza and soybean, intercropping sugarcane + mycorrhiza and soybean + rhizobium, intercropping sugarcane + mycorrhiza and cowpea + rhizobium. Combined analysis of variance showed that different treatments were significant on yield of sugarcane and harvest index of sugarcane, land equivalent ratio (LER), cowpea seed yield, number of pods in cowpea and soybean, weight of 1000- grain of cowpea, number of nodules and dry weight of nodules in cowpea root. Different locations also had a significant effect on yield and harvest index of sugarcane, cowpea seed yield, number of pods in cowpea, weight of 1000- grain of cowpea, number of nodules and dry weight of nodules in cowpea root and harvest index of soybean. The K factor in cowpea and soybeans was significantly difference in different places, but there was no significant difference in sugarcane. In general, radiation use efficiency in intercropping treatments was better than pure crop and the highest radiation use efficiency in sugarcane (2.21 g.Mg-1) and the most economic yield of sugarcane (111.6 t/ha) and the most seed yield of cowpea ( 2.27 t/ha) were in intercropping sugarcane + mycorrhiza and cowpea + rhizobium which indicates good coexistence of sugarcane root with mycorrhiza and the root of cowpea with rhizobium. The highest LAI (4.612) and the highest K factor (0.595) in cowpea were obtained in cowpea + rhizobium. The highest LAI (6.713) and the highest K factor (0.473) in sugarcane were obtained in intercropping sugarcane + mycorrhiza and cowpea + rhizobium. The results showed that the highest water productivity (4.46 k/m3), the highest sugarcane stem height (215.6 cm), the largest diameter in the middle (41 mm) of the stem and at the bottom (43.88 mm) of the stem, the highest dry weight roots (269.8 g) and the highest brix percentage (21.88 %) of sugarcane stem extract and the highest harvest index for cane yield (93.81 %), highest harvest index for sugar yield (7.34 %), purity (87.28 %), white sugar percentage (11.73 %), and maximum nitrogen (2.1 %), phosphorus (0.26 %), potassium (1.91 %) in the lamina, and moisture content (83.82 %) in the leaf sheath of sugarcane were related to intercropped sugarcane + mycorrhizal and cowpea+ rhizobium treatment. The amount of LER in all treatments was higher than one, indicating the beneficial effects of intercropping. The most LER (2.068) was related to intercropped sugarcane + mycorrhizal and cowpea treatment. Weeds were well controlled without using herbicides in intercropping treatments. Therefore in this study the best results were obtained in sugarcane crop (inoculation with mycorrhizal) and cowpea (inoculation with rhizobium). The results of this experiment showed that the synergistic effects of mycorrhizal fungi and cowpea on the traits were positively evaluated on the traits. Therefore, the use of mycorrhizal and rhizobium and the use of legumes in intercropping, improved soil fertility, increased land use efficiency and radiation. Intercropping through non-chemical control of weeds reduces the risk of pollutants in the environment. Therefore, in order to improve the qualitative and quantitative properties of sugarcane, intercropping sugarcane with legumes, such as cowpea, and also inoculation of sugarcane cuttings during cultivation with mycorrhiza fungi is recommended.