Abstarct: The external application of compounds such as vitamins and melatonin have been tested to reduce the negative effects of environmental stresses on the plant. Melatonin and vitamins B group has a significant role as a inhibition of reactive oxygen species, antioxidant activity in plant. In this regard, the effect of these materials on physiological, agronomical and qualitative aspect of soybean was investigated. This study consists of three experiment ; laboratory, pot and field were carried out at the department of agronomy and plant breeding of shahrood university of technology in 2017 on soybean. In laboratory, was conducted as a factorial experiment baxsed on completely randomized design with three replications in germinator. Soybean seeds were pretreated with melatonin (0, 0.1 and 0.2 mM) and vitamins B group (0, thiamine, riboflavin, niacin, pantothenic acid, and pyridoxine 100 mg / l) for 10 hours. Pot and field was conducted as a factorial experiment baxsed on completely randomized block design with three replications. Treatments including foliar application melatonin (0, 0.1 and 0.2 mM) and vitamins B group (0, thiamine, riboflavin, niacin, pantothenic acid, and pyridoxine 100 mg / l). Foliar application was done in the pot and field experiments 60 days after planting with 2 interval days. 15 days after treatment, the samples and theire charactristic were recorded. In the labratory, pretreatment of melatonin and vitamin b significantly increased shoot length, seedling dry weight, root dry weight and seedling vigor weight index. In most of traits, the combination of 0.2 Mm melatonin and pantothenic acid showed the most positive effect. In the field and pot experiment, interactions between the foliar application of melatonin and vitamin B increased chlorophyll b, carotenoids, relative leaf water content, plasma membrane stability, flavonoid, and soluble sugar content. The maximum yield was obtained in the combination of treatment with the highest concentration of melatonin and pantothenic acid, which showed an increase of approximately 3.5 times that of the control plants. The effect of melatonin in the non-presence of vitamin b, the percent of protein and oil content increased. Vitamins B also increased seed oil percent compared to non- application treatment. The highest value of seed oil percent was obtained with pantothenic acid and pyridoxine which had no significant difference. In the pot, foliar application of melatonin and vitamin B enhanced the plant protective system and increased the detoxification of reactive oxygen species by reducing the accumulation of hydrogen peroxide, malondialdehyde and increasing the activity of antioxidant enzymes. The maximum activity of superoxide dismutase and minimum accumulation of hydrogen peroxide were recorded in the plants that received the highest combination of melatonin and pantothenic acid. Finally, in the research area, pantothenic acid with 0.2 mM melatonin had more effect on traits and can be described as the best treatment combination.