Abstarct: The purpose of this study was to encapsolate mortierella alpina lipid using chitosan nanogel, and to investigate the effect of the coating on the oxidative stability of mortierella alpina lipid. The results of the gas chromatography test showed that about 42% of the mortierella alpina lipid was composed of arachidonic acid that has 20-carbon fatty acid with 4 unsaturated bonds. The results of the FT-IR spectrum confirmed the conection between chitosan and capric acid as well as stearic acid. The results of scanning electron microscopic photos showed that with the addition of stearic acid and capric acid, chitosan chains appropriately formed nanoparticles, and improved the formation of chitosan-stearic acid nanoparticles compared to chitosan-capric acid. The effect of each variable factors like chitosan, stearic acid and capric acid on the size of oil droplets and the stability of Pickering emulsion mortierella alpina lipid was investigated in three hours and seven days after production. The results showed that there was not significant difference in the level of 5% between chitosan, chitosan-stearic acid and chitosan-capric acid nano-gels in terms of particle size. Afterwards, the oxidative stability of the emulsion of mortierella alpine lipid during 20 days at ambient temperature was evaluated using peroxide test. The results showed that emulsion produced by chitosan-stearic acid nanogel was the most oxidative stability. The results of rheological tests showed that viscosity behaviors were dominated by elastic behaviors in the impregnating emulsion with unmodified chitosan at the applied frequencies, and the elastic behavior of the emulsion sample prepared with chitosan-stearic acid was slightly higher than the emulsion prepared with chitosan-capric acid nano-gel. The results of contact angle measurement showed that the chitosan-stearic acid powder emulsion had the highest contact angle and had the lowest water absorption than other samples.