Abstarct: Genetic diversity DNA key identification and introducing superior walnut genotypes is very important in Iran as one of the main producers of walnut and the origin of this plant. In 2001, walnut collection germplasm estabolished semnan Research Center (Shahrood) from seeds of selected walnut genotypes important Iranian walnut areas. From these seedlings, 21 genotypes were selected with well pomological characteristics and were grafted onto seedlings. The morphological and pomological characteristics genetic variation of these genotypes, was investigated with 10 ISSR primers and 6 SSR primers pair. Nut weight and kernel weight were 17.5g and 10.3g respectively in OR23 genotype. The average kernel percentage of selected walnuts was 51.5%. The highest kernel percentage (62.7%) was observed in T12 genotype. The shell thickness of the K26, T1, and OR26 and R2G8 genotypes was thick which reduced marketing of this genotype. Of the ten ISSR primers, 112 fragments were produced in 21 walnut genotypes, 102 of them were polymorphism and used for genetic diversity analysis. The highest bands number of was observed in UBC.826 and UBC.888 primers with 14 bands. The highest Rp was 7.71 in UBC.826 primer and then in UBC.887primer. The similarity coefficient between walnut genotypes varied from 0.51 to 0.88. Dendrogram divided 21 walnut genotypes into two main groups and three subgroups in the first group, which consistently matched the results. The six SSR loci, a total 32 alleles were obtain that the highest allele number,8, was observed in WGA009. The identification key was created baxsed on the loci for 21 walnut genotypes. WGA009 and WGA276 were able to identification 19 genotypes from 21 genotypes.