Abstarct: Stevia rebaudiana from Asteraceae has sweet-tasted leaves due to the presence of diterpene glycosides, which are not being absorbed by the body. Thus, it bears no calory and can be used as an alternative sweetner. However, the sweet extract of stevia has a limited market share because of the bitter taste of stevioside. Here, a construct was built within pHANNIBAL vector containing partial sequences homologous to SrUGT85C2. A gene involved in the mextabolic pathway of stevioside. in sense and antisense orientation. The aim is to post-transcxriptionally silence the gene via RNAi and seek any organoleptic changes: reducing the undesirable bitter taste. For this purpose, two pairs of primers were designed to amplify both fragments. Following cloning of each sense and antisense in pTG19-T vector, the fragments were sequenced and excised with corresponding endonucleases. The restricted fragments were cloned sequentially inside the pHANNIBAL and the integrity of the engineered plasmid was checked via DNA sequencing.