Abstarct: Blueberry (Vaccinium corymbosum L.) is a perennial tetraploid plant. It is belongs to Ericaceae family and the genus Vaccinium. This plant is native to North America. Due to its benefits on human health and its antioxidant components, including flavonoids, anthocyanins and phenolic acids, recent demand for blubberry has been increased. In this regard, tissue culture can be the most appropriate method to respond to this increased demand and reproduction of this plant. The aim of this research was to optimize the micro proliferation of this plant. For this purpose, In vitro micro-samples of three different blubbery varieties including Bluecrap, Chandler and Reca were prepared and cultured in a WPM culture medium along with different concentrations of zeatin and dikegulac hormones and after two months, the length and number of lateral branches were measured. In addition, rooting potential was investigated in sugar and IBA-enriched WPM culture media, with or without active charcoal. Results were analyzed in a completely randomized factorial design. The tallest lateral branches were obtained using the concentrations of ۰.۶ and ۰.۴ mg / L of zeatin and no use of dikegulac in the Chandler and Bluecrap varieties, respectively. While, the shortest lateral branches were obtained in the absence of zeatin and dikegulac in the Chandler variety. The highest numbers of lateral branches were observed using the hormonal combination of ۰.۸ mg / L zeatin with ۱۵ mg / L dikegulac and the lowest numbers of ones were achieved in no use of zeatin and different levels of dikegulac. Application of sucrose and IBA hormone had no significant effect on rooting of different varieties. In addition, rooting induction of cultivars without activated charcoal treatment was not performed, but it was done in the presence of charcoal activated. Totally, the results of this study showed that zeatin hormone had positive effects on proliferation and growth of blubbery varieties While, dikegulac did not significantly effect on the growth of blueberry varieties. Also, it was found that the use of activated charcoal was essential for Stimulation of rooting in blubbery varieties.