Abstarct: In order to evaluate the effect of weed management and the separate and co-inoculation of Rhizobium and Mycorrhiza with the use of chemical fertilizer, on quantitative and qualitative characteristics of soybean (Glycine max L.) cultivar Katoul, a factorial experiment was conducted in a randomized complete block design with three replications in a farm in Ali Abad Katoul during the years of 2015-16. Treatments were included 1- Weed management at two levels of weed free and weedy check, 2- inoculation of biological fertilizer at four levels of non-application (control), inoculation of seeds with Bradyrhizobium japonicum, inoculation of seeds with mycorrhizal fungus Glomus mosseae and co-inoculation with Mycorrhiza and Rhizobium and 3- utilization of nitrogen fertilizer (pure) from the source of urea fertilizer at three levels of zero (control), 25 and 50 kg / ha. The results of this experiment showed that increasing the utilization of nitrogen fertilizer in both weed free and weedy check treatments, increased the maximum leaf area index, but this increase was higher during weed free. Also, the results of this experiment showed that increasing the utilization of nitrogen fertilizer, inoculation with biological fertilizers and controlling weeds increased the number of pods per plant, the weight of 100 seeds and the number of seeds per plant. The results showed that the number of pods per plant increased by 13% in the second year compared to the first year of the experiment. baxsed on the results of the mean comparison of the first year of experiment, weed free and weedy check treatments, the number of pods in soybeans 49.41 and 38.97 pods per plant respectively, which had a significant difference compared to each other. In the second year, weed free treatments showed an increase of 27% in number of pods per plant than weedy check treatments. This result showed the high impact of weeds on the number of pods per plant, which is the most important yield components of soybean. The weight of 100 seeds of soybeans increased with increasing utilization of chemical fertilizers. so that the weight of 100 seeds of soybeans at 0, 25 and 50 kg/ha of application of fertilizer per hectare was 16.1, 17.2 and 18.2 gr respectively. The results of the mean comparison showed that in the second year of the experiment, biological yield was significantly higher than the first year. so that the biological yield in the first and second years was 8866.78 and 9544.88 kg/ha respectively. The results showed that chemical fertilizer treatment at 50 kg/ha had a 16% impact on biological yield of soybean compared to non-fertilizer treatment. The results of grain yield showed that at 0, 25 and 50 kg/ha nitrogen fertilizer yields were 3531.89, 3948.62 and 4160.80 kg /ha, respectively. The number of bacterial nodules in the second year of experiment (44.17 nodule per plant) was significantly higher than the first one (35.15 nodule per plant). In addition, the co-interaction of biological and chemical fertilizer showed that the highest dry weight of the nodules (13.38 mg) was associated with the co-inoculation of Mycorrhiza and Rhizobium, and the non-usage of chemical fertilizer, and the lowest dry weight of the nodules with 7.97 mg, respectively, related to non-usage treatments of biological fertilizers and consumption of 25 and 50 kg/ha chemical fertilizer. The study of weeds seed condition in the seed bank showed that the highest number of seeds observed in the seed bank belonged to Echinochloa crusgali, in both years of experiment. Additionally, in the first year of experiment, in biological fertilizer treatments, the highest number of weed seeds (70.4) belonged to the co-inoculation treatment and the least amount with (41.2) was for inoculation with rhizobium. These results indicate that the effects of the treatments on the studied traits are significant. Evaluation of qualitative factors showed that treatments did not have a significant effect on seed oil percent, but affected seed protein percent. baxsed on the results of the mean comparison, co-effect of weed and chemical fertilizer interaction show that in both weed free and weedy check tratments protein percent increased with the use of chemical fertilizer compared to the control.