Abstarct: food security and health is one of the most important issues of human life. The excessive use of fertilizers and chemical pesticides, in addition to economic problems, entails irreparable damage to the environment and ecosystems and, increase in diseases such as cancer. Cowpea Vigna unguiculata L. is an important grains that cultivated in wide area in the world and consume as a food source. One of the most important measures to increase the effect or optimize the use of acaricides is to use them at appropriate dosage and in appropriate temperature and relative humidity conditions. Tetranychus urticae, Koch, is one of the most cowpea, which reduces the quality and quantity of the product. In this study, in order to determine the best conditions for the maximum mortality rate, with the aim of maximum mortality of adult female mite, the process input variables were considered in the range of Temperature 25-30 ° C, humidity 55- 65%, and dosege 200 -1260 microliters per 1000 cc distilled water of fenpyroximite acaricide , Temperature 25-30 ° C, humidity 55- 65%, and dosege 900 -2300 microliters per 1000 cc distilled water of propargite acaricide , Temperature 25-30 ° C, humidity 55- 65%, and dosege 69 -2330 microliters per 1000 cc distilled water of Fenazoquine acaricide. The leaf disk of cowpea was used to carry out Tetranychus urticae bioassay tests. To determine the optimum conditions, the maximum mortality rate of adult female mites from the central composite design (CCD) and (RSM) method Design Expert 7.00 software were used. The results of analysis of variance indicated and the linear equation proposed of effect dosage factor and humidity were significante. The effect factor of temperature on the mortality of the whole female mites was not significante for Fenpyroximite acaricide. According to the linear equation proposed and effect of temperature and humidity on the mortality of the whole female mites was significante for acaricide propargite . linear equation proposed of effect factor humidity was significante for Fenazoquine acaricide. the best point optimum maximum for female mites mortality 21% - 22% in humidity is 57.03% and the dosege of 1045/ 14 ml per 1000 ml distilled water. The ideal optimum maximum for female mites mortality 12/55 % - 14/ 6% propagitis acaricide was determined in temperature of 28/99 ° C, humidity 62/97 %,. the optimum conditions for Fenazoquine with female mites mortality 8/5% - 9/2% and 527.30 microliters per 1000 cc distilled water.