Abstarct: Shoot organogenesis from mature leaf and petiol tissues of two Pelargonium ×domesticum cultivars, ‘Aristo’and ‘Bermoda’, grown in the greenhouse, were optimized in the presence of thidiazuron (TDZ) and BA. In the first experiment, the effect of different concentrations of 6-benzyladenine (BA) alone or compounded with Indole-3-acetic acid (IAA) on basal Murashige and Skoog (MS) medium were evaluated with two subculture for shoot induction from lateral bud explants in stablishment. The results showed that the highest regeneration percentage and shoot number in ‘Aristo’ (100 %, 42.3) and ‘Bermoda’ (82.33%, 20.89) obtion 2 mg/l BA in first subculture. However, maximum shoot mass and leaves number (195.7 mg, 8.16) was achieved in control plants (with one plantlet per explant). In the second subculture, highest regeneration and shoot number (100 %, 42.78) in ‘Aristo’ and ‘Bermoda’ was recorded with 1 mg/l BA. Control plants (with one plantlet per explant) showed highest number of leaves (6.99) on MS media. Maximum shoot mass (216.1mg) was achieved with 1 mg/l BA compounded with 0.5 mg/l IAA . In the second experiment, The effects of types of two Pelargonium×domesticum cultivars, ‘Aristo’and ‘Bermoda’ and different concentration of plant growth regulators was evaluated on direct regeneration from mature leaf and petiol explants. Frequency of regeneration reached approximately (85.2 %) for both cultivars on basal Murashige and Skoog (MS) medium supplemented with 2 mg/l TDZ, 2 mg/l of 6-benzyladenine (BA) and 1 mg/l a-naphtaleneacetic acid (NAA) for a period of 2 weeks and followed by subculture of explants to a fresh medium containing 2 mg/l BA and 1 mg/l NAA with the induction of shoots per explant (25/61). however the highest shoot length obtion with 1 mg/l BA with 1 mg/l NAA only at 33.25 mm.In third experiment, Regenerated plantlets were rooted on half-strength MS medium supplemented with IAA and IBA. Maximum rooting percentage and shoot mass and root mass was achieved with 2 mg/l of IAA and 1/5 mg/l of IBA and maximum number of root with 1.5 mgl of IBA and highest root length (200.03 mm.) were obtained with 2 mg/l of IAA. regenerated shoots from both cultivars developed roots when transferred to organic soil mix with cocopite and perlite (1:3:1) , acclimatized, and successfully transferred to greenhouse conditions. frequency of survival was 100 % for ‘Aristo’ and ‘Bermoda’ in 2 mg/l IAA and 1/5 mg/l of IBA treatments. The present study provides a comprehensive methodology for high efficiency micropropagation of Pelargonium ×domesticum for commercial production.