Abstarct: This research was carried out to investigate the effect of spermidine and drought stress (irrigation interval) on chickpea growth and yield in a research farm of Shahrood University of Technology in 2016 as split plot baxsed on randomized complete block design with three replications, in which drought stress (irrigation interval) was distributed in the main plots and subplots were allocated to spermidine. Drought stress levels (irrigation) included control (7-day irrigation interval; D0), mild stress (10-day irrigation interval; D1) and severe stress (13-day irrigation interval; D2), and spermidine levels including control (ordinary water spray on plant; SP0), 0.3 mM (SP1) and 0.6 milimolar spraying. No plant tension was induced Until plant establishment, no stress was imposed on plant. Spraying was carried out at (a), four-leaf, (b) flowering, and (c) seed milky stages. Some of the traits studied included yield and yield components, biological yield, pod number per plant, leaf chlorophyll and activity of antioxidant enzymes guaiacol peroxidase and catalase. The results showed that drought stress (increasing irrigation intervals) reduced the grain yield and other traits, while the 13-day irrigation interval increased the percentage of protein. Spermidine could reduce the negative effect of drought stress on many traits including grain yield. It should be noted that the interactive effect of stress (irrigation interval) and spermidine was significant on most of traits like biological yield, grain yield, 100-grain weight and leaf chlorophyll.