Abstarct: In recent years, due to the importance of secondary mextabolites in medicinal plants, it is important to find the conditions that can have the most impact on the economic production of these compounds. In the present study, the effect of salicylic acid and methyl jasmonate as chemical elicitors in three concentrations (50, 100, 150 µM), and the effect of UV-A and UV-B radiation as physical elicitors in two levels (30 and 60 minutes) on callus of Salvia lerrifolia Benth was tested in a completely randomized design with three replications. The highest of fresh and dry weight, content of total phenol and flavonoids of callus were observed in the samples in which treated with 100 μM methyl jasmonate. By increasing the concentration of methyl jasmonate from this amount, content of total phenol, flavonoids, rosmarinic acid and caffeic acid decreased. The accumulation of these compounds increased as increasing salicylic acid concentration. The highest accumulation of rosmarinic acid was observed in 150 µM salicylic acid, while 50 µM salicylic acid had the highest effect on increasing the production of caffeic acid. The content of caffeic acid decreased as increasing salicylic acid concentration. UV-A and UV-B radiation as elicitors increased content of total phenol, flavonoids, rosmarinic acid and caffeic acid in calluses. However, the accumulation of these secondary mextabolites was decreased as increasing treatment time from 30 to 60 minutes in both types of radiation. The results showed that UV-B radiation for 30 minutes had a greater effect on increasing the content of caffeic acid and rosemarinic acid. According to the results, salicylic acid has the most effective on increasing the accumulation of rosmarinic acid and caffeic acid in leaf calluses compared to other treatments. Thus by optimizing its concentration, the content of this secondary mextabolites can be increased.