Abstarct: The main objective of sustainable agriculture is to reduce the inputs and improve the soil nutrients cycling by reducing soil cultivation and using biological fertilizers instead of chemical fertilizers, in order to increase the yield of agricultural products. In order to investigate the effect of mycorrhiza on root and shoot morphology of two forage sorghum cultivars and soil bacterial quality under irrigation with municipal wastewater, a factorial experiment in a randomized complete block design with four replications was conducted in 2017 in greenhouse of Islamic Azad University of Sabzevar. The experimental factors included bio-fertilizer at two levels: application and non-application of arbuscular mycorrhizal fungi, irrigation at three levels: regular water, wastewater and regular water and wastewater alternatively and two cultivars of sorghum: Speedfeed and Syahzan Sabzevari. The results showed that irrigation with wastewater significantly increased leaf chlorophyll index, chlorophyll b content, root dry weight, shoot dry weight and plant phosphorus content compared to control. The wastewater application caused a significant increase in the total root length, root area and root volume in Syahzan cultivar, but had no significant effect on the root length and root area of the Speedfeed cultivar. The use of wastewater reduced the percentage of digestible dry matter, crude protein, cell wall and insoluble fiber of forage as compared to control treatment. Inoculation of mycorrhiza increased the percentage of digestible dry matter and soluble sugar in water compared to the control. Mycorrhiza decreased the digestible dry matter and increased the percentage of cell wall and insoluble fiber percentage in Syahzan cultivar. The percentage of water-soluble sugar was not affected by mycorrhiza. The highest amount of raw fiber was observed in Syahzan cultivar. Inoculation of mycorrhiza had no effect on total root length, root are, root volume and root dry weight in Syahzan cultivar, but decreased these traits compared to non-inoculated control in Speedfeed cultivar. The concentration of phosphorus in the Syahzan cultivar was significantly higher than that of Speedfeed. The highest content of chlorophyll a was obtained in Syahzan cultivar and in the presence of mycorrhiza. The use of regular water and wastewater alternatively reduced the total coliform and fecal coliform of the soil under conditions of use of Syahzan cultivar. The total fecal coliform of the plant and total coliform and fecal coliform of the soil decreased in terms of the use of regular water and wastewater alternatively and inoculation of mycorrhiza. Non-inoculation of mycorrhiza caused a significant increase in total coliform of the plant in Syahzan cultivar. The number of infectious bacteria in the soil decreased over time. The use of mycorrhizal fungus increased the rate of reduction in the amount of fecal coliform in the soil. According to the results of this experiment, the use of regular water and wastewater alternatively is recommended in the Syahzan Sabzvari local cultivar. Also, due to the improvement of sorghum growth characteristics in irrigation with wastewater and without using mycorrhiza, the simultaneous use of biological fertilizers and wastewater is not recommended.