Abstarct: Wheat germ oil has the highest tocopherol content among the vegetable oils and the highest levels of polyunsaturated fatty acids of high nutritional value. However, unsaturated fatty acids reduce the oxidative stability of wheat germ oil. The present research aimed to produce nano lipid carriers containing wheat germ oil in order to enhance its oxidative stability. The results showed that the best formula for preparation of nano lipid carrier particles containing wheat germ oil in terms of size is the use of 407 poloxamer emulsifier and precirol solid lipid phase. The 1:1 ratio of poloxamer emulsifier to lipid phase led to the production of particles sized 52.7 nm. Due to non-polarity of the poloxamer emulsifier used in this study, the zeta potential of particles was close to zero. Stability of particles during 15 days of storage at room temperature was very good. Studying the thermal behavior of the formula with the 1:1 ratio of poloxamer emulsifier to lipid phase indicated that the emulsifier, solid phase, and liquid phase were mixed with each other very well and created a new structure. The results obtained from the study of nano lipid carrier’s oxidation during 35 days of storage showed that oxidation changes in nano lipid carriers were lower than the changes in the emulsifier. The best formula for oxidative stability was the formulation containing the 2:1 ratio of poloxamer emulsifier to lipid phase. In addition, the amount of peroxide in nano lipid carriers prepared with poloxamer emulsifier at acidic pH was slightly lower than its amount at alkaline pH. Hence, these nano carriers are recommended to be used in acidic formulations.