Abstarct: The Greater Wax moth is one of the most important pest of stored products and beehives. Chemical pesticides are used to control it. Inappropriate using of pesticides caused serious environmental problems and also chronic effects on human health. Pest damage is reduced by the optimum use of these materials.The purpose of optimum use is select the correct pattern and applying appropriate methods for use toxin. In this study in order to determine optimal points to achieve maximum mortality with minimum dosage and the effect of factors such as temperature (25-35°C), humidity (70-80 %) and the dosage (1500-2000 µL) for chlorpyrifos and (4000-6000 µL) for palizin and (200-500 µL) for deltamethrin in the mortality of fifth instar larvae of greater wax moth were applied response surface method. The experiments were performed according to central composite design. Temperature and dosage had significant effect on mortality of fifth instar larvae of greater wax moth. The Optimum conditions in minimum dosage to obtain the maximum mortality 7.9 fifth instar larvae of greater wax moth are: 25 ° C, humidity 80% and 1500 microliter of chlorpyrifos in the 1000 cc of water and for palizin 7.4 fifth instar larvae of greater wax moth are: 25 ° C, humidity 80% and 4000 microliter in the 1000 cc of water and for deltamethrin 7.6 fifth instar larvae of greater wax moth are: 25 ° C, humidity 70% and 200 microliter in the 1000 cc of water. Results of experiment show that linear and quadratic effect of temperature and dosage of chlorpyrifos and linear effect of palizin and deltamethrin on mortality of fifth instar larvae of greater wax moth.