Abstarct: Echinacea (Echinacea purpurea) is an important medicinal plants used in pharmaceutical industry and immunostimulants. hairy roots, the results of genetic transformation by Agrobacterium rhizogenes bacterial DNA (T- DNA) from the bacterial Ri (Root inducing) plasmid in plant genom. now these bacteria are widely used to perform the transformation and development of transgenic roots. reference the importance of medicinal plants Echinacea purpurea hairy root growing systems is necessary. In this research the amount of phenolic compound, contents of polysaccharides and induce hairy root percentage of the leave and hypocotyle explants were determined in hairy root induced by A4, A13, 15834 and 15834 containing the binary vector pbi121- GUS strains of A. rhizogenes, as well as the control (untransformed) roots. The results showed that the induction of hairy roots of Echinacea purpurea herb leaves of inoculated strains A13,15834, A4 and AR15834- PBI121 respectively 60%, 40%, 55% and 0, and at hypocotyl explants of 85%, 45%, 70% and 75% respectively and the significant difference is between types strain bacterial and explants hairy roots was performed in PCR reactions and GUS histochemical localisation confirmed. The accuracy of the results of the PCR test for gene amplification 780 bp rolB and 320 bp to the GUS reporter gene in transgenic roots showed. The results indicated a significant increase of total phenolics in hairy roots of strains was investigated. As well significant increase of polysaccharide content in hairy root induced by strains 15834, A13 and significant decrease of these compounds in hairy roots induced by A4 strain. In this research we studied the efficiency of regeneration of root hairy. Results showed that MS medium containing 2 mg.l-1 NAA and 0/25 mg.l-1 BAP after 3 weeks of callus formed. Ri plasmid T-DNA transfer and entry GUS gene into the genome of transgenic plants using PCR with primers specific for the GUS gene and the histochemical GUS assay was confirmed.