Abstarct: During recent decade, the use of chemical inputs in agricultural lands has caused some main environmental problems such as fertility reduction, soil salinity, imbalance of mextals in soil and contamination by some heavy mextals. This study was carried out to investigate the effect of different concentrations of salinity and chromium on soil chemical characteristics and qualitative and quantitative properties of Momordica charantia in presence of mycorrhizal fungi Glomus versiform, as a factorial experiment in a randomized complete block design with three replications in Shahrood university research greenhouse. The experiment factors were included salinity of sodium chloride at three levels 0 (control), 4 and 8 dS/m, chromium at three levels 0 (control), 10 and 20 mg Cr/kg soil as potassium dichromate and mycorrhizal fungi Glomus versiform with two levels without inoculation (control) and inoculation with 100 g per 8 kg soil. The results showed that the effect of the main treatment of salinity was significant (p≤ 0.01) on some of characteristics of plant such as photosynthetic pigments (except carotenoid), enzymes antioxidant, plant fresh weight and chemical characteristics such as electrical conductivity and acidity. The intraction effects of salinity - chromium and mycorrhiza - chromium were significant (p≤ 0.01) on catalase activity and guaiacol peroxidase activity. In this research, the increase of application of chromium was caused to decrease the photosynthetic pigments (except anthocyanin) and plant growth parameters (plant fresh and dry weights). The inoculation with mycorrhizal fungi was caused to prevent the negative effects of chromium and salinity stresses on some of characteristics such as the amount of root carbohydrate, carotenoid, phosphorus and chromium content of plant. Also, the intraction effects of salinity - chromium - mycorrhiza were significant on catalase and guaiacol peroxidase activities, in this research. So using the appropriate amounts of mycorrhizal fungi, in areas where the amount of chromium is critical, can increase the yield compared to the chemical fertilizers and expensive methods and also prevent contamination of soil and groundwater.