Abstarct: Chemical fertilizres used in agriculture are sources of environmental pollutants specially groundwater and soil recources. Using of living organisms and organic compounds adsorbing pollutants in soil is a new method to prevent pollution of this waters. Accordingly, an experiment was conducted to evaluate the effects of biochar and arbuscular mycorrhiza symbiosis with corn on reduction of nitrate leaching. A factorial randomized complete block design with three replications was performed under a greenhouse exprement. Factors were tested including three biochar levels (including without biochar (B0) and biochar (B1) and biochar (B2)), three mycohrriza levels including (without mycohrrizae (M0) and Funneliformis intraradices (M1) and Funneliformis versiforme (M2)) and two levels urea fertilizer levels ( including (without fertilizer (N0) and with fertilizer (N1)). The results showed that the application of mycohrriza had significant effect on plant height and shoot dry weight but biochar alone had no significant effect on these characteristics. However, its co-application with mycorrhiza and fertilizer had a positive effect on some of these characteristics. Mycorrhiza inoculation and application of biochar, had significant effect on reducing the leaching of nitrate and ammonium and their storage in soil. The other results also showed that the effects of double and triple-tested factors were significant on growth characteristics, nitrate and ammonium in soil and leaching of soluble. Regarding to the proper function of mycorrhiza and biochar to reduce nitrate and ammonium leaching, it can be concluded that mycorrhiza and biochar can cause positive effects on traits. In fact, according to these results, the use of mycorrhiza as a bio-fertilizer instead of chemical fertilizers, can reduce the leaching of this element. In general, mycorrhiza can be used as a replacement for chemical. Appropriate amounts of nitrogen fertilizers along with mycorrhiz and biochar, could increase yield compared to the chemical fertilizers alone. The results could be useful to reduce fertilizer applications, decrease production cost and to prevent contamination of soil and groundwater.