S188 : Evalution of retrotransposons as molecular markers for genetic diversity study of apricot genotypes.
Thesis > Central Library of Shahrood University > Agricultural Engineering > MSc > 2014
Authors:
Anahita Panji [Author], Shahrokh Gharanjik[Supervisor], Naser Farrokhi[Supervisor], Babak Abdolahi [Advisor], Mohammad Abedini [Advisor]
Abstarct: Retrotransposons are current component of plant genomes. Ubiquitous, activity and abundant of retrotransposons thorough the plant genome, make them useful molecular marker. We used inter-retrotransposon amplified polymorphism (IRAP) and retrotransposon-microsattelite amplified polymorphism (REMAP) markers to assess genomic diversity levels and survey the activity of LTR retrotransposon elements in 23 cultivars apricot. In this study out of 11 combined primers, 9 primers produced scorable and polymorphic banding patterns. This 9 primers produced 168 bands so 168 bands showed acceptable polymorphism. The number of replicated fragments by different primers were varied, the highest number of fragments was 26 belong to REMAP (LTR6+P9) primers and the lowest of them was 11 belong to IRAP (LTR10) primer. The highest Shannon's variation coefficient and observed heterozygosis belong to LTR13 primer from IRAP primers so that probability indicated IRAP marker with regard to other studied markers was better.
Keywords:
#apricot #retrotransposon #genetic diversity #IRAP #REMAP #cluster analysis #Principal Component Analysis Link
Keeping place: Central Library of Shahrood University
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